Genetic Transformation in Cucumber as Influenced by Inoculation Time and  Co-cultivation Period

Dr. S. M. Faisal; Dr. M. S. Haque; Dr. K. M. Nasiruddin; Dr. N. F. Rownok

doi:https://doi.org/10.51976/ijari.1122312

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Genetic Transformation in Cucumber as Influenced by Inoculation Time and Co-cultivation Period

S. M. Faisal, M. S. Haque, K. M. Nasiruddin, N. F. Rownok

Vol 11 , Issue 2 , April - June 2023 | Pages: 81-89 | Research Paper

https://doi.org/10.51976/ijari.1122312

Received: April 30, 2023 | Revised: May 25, 2023 | Accepted: June 05, 2023 | Published Online: June 15, 2023

Author Details ( * ) denotes Corresponding author

1. * S. M. Faisal, Principal Scientific Officer, Faculty of Agriculture,, BARI, Ramgarh, Khagrachari hill district, Bangladesh (osru2002@yahoo.com)

2. M. S. Haque, Professor, Faculty of Agriculture, Department of Biotechnology, BAU, Bangladesh

3. K. M. Nasiruddin, Professor, Faculty of Agriculture, Department of Biotechnology, BAU, Bangladesh

4. N. F. Rownok, Student, Faculty of Agriculture,, IUBAT, Bangladesh

To study the effect of inoculation time and co-cultivation periodin genetic transformation for abiotic stress resistance in cucumber (var. Shital), leaf, nodal and internodalcalli were subjected to Agrobacterium tumefaciens mediated transformation using LBA4404 strain containing CIPK sense gene. Transformation ability was examined by histochemical assay of GUS reporter gene in survived calli. Conspicuous GUS positive (blue colour) region were detected in callus tissue. There were 3 factors in this investigation. Factor A consisted of three types of explants viz. leaf, nodal and internodal callus, factor B consisted of two durations of inoculation time viz. 3 and 5 min and factor C consisted of two co-cultivation periods viz. 24 and 48 hours. Both number and percentage of GUS positive callus were higher (3.17 and 52.87 %, respectively) when callus were kept in bacterial suspension for higher time (5 min) and were lower (2.15 and 35.88 %, respectively) when they were soaked in bacterial suspension for minimum time (3 min). The longer co-cultivation period (48 hrs) showed better performance than shorter one. The higher number and percentage of callus (12.45 and 51.87 %, respectively) were survived when they were kept in co-cultivation medium for two days (48 hrs). The highest number and percentage (4.04 and 67.38 %, respectively) GUS +ve callus obtained from leaf explants when they were immersed in Agrobacterium suspension for 5 min and following immersion transferred into cocultivation media for 48 hrs.

Keywords

Cucumber, genetic transformation, abiotic stress

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